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Document type:
journal article 
Author(s):
Mykhaylyk, Olga; Sanchez-Antequera, Yolanda; Vlaskou, Dialechti; Cerda, Maria Belen; Bokharaei, Mehrdad; Hammerschmid, Edelburga; Anton, Martina; Plank, Christian 
Title:
Magnetic nanoparticle and magnetic field assisted siRNA delivery in vitro. 
Abstract:
This chapter describes how to design and conduct experiments to deliver siRNA to adherent cell cultures in vitro by magnetic force-assisted transfection using self-assembled complexes of small interfering RNA (siRNA) and cationic lipids or polymers that are associated with magnetic nanoparticles (MNPs). These magnetic complexes are targeted to the cell surface by the application of a gradient magnetic field. A further development of the magnetic drug-targeting concept is combining it with an ult...    »
 
Journal title abbreviation:
Methods Mol Biol 
Year:
2015 
Journal volume:
1218 
Pages contribution:
53-106 
Language:
eng 
Print-ISSN:
1064-3745 
TUM Institution:
Institut für experimentelle Onkologie und Therapieforschung