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Document type:
Zeitschriftenaufsatz 
Author(s):
P. Fraga García; M. Brammen; M. Wolf; S. Reinlein; M. Freiherr von Roman; S. Berensmeier 
Title:
High-gradient magnetic separation for technical scale protein recovery using low cost magnetic nanoparticles 
Abstract:
Downstream processing still lacks efficient and integrated separation techniques. We present a high-gradient magnetic separation (HGMS) process for the successful purification of recombinant histidine-tagged Green Fluorescent Protein (His-GFP) from an E. coli cell lysate by means of superparamagnetic iron oxide nanoparticles functionalized with a pentadentate chelate ligand. The separator is an improved rotor stator prototype with 1 L chamber volume. Using 100 g of carrier, a purification pe...    »
 
Keywords:
High-gradient magnetic separation; Protein recovery; Downstream processing; Magnetic nanoparticles; Large-scale purification 
Journal title:
Elsevier 
Year:
2015 
Year / month:
2015-06 
Reviewed:
ja 
Language:
en 
Accepted:
19.06.2015 
Date of publication:
19.06.2015 
TUM Institution:
FG für Selektive Trenntechnik 
Ingested:
14.07.2015