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Document type:
journal article 
Author(s):
Rowinska, Zuzanna; Gorressen, Simone; Merx, Marc W; Koeppel, Thomas A; Liehn, Elisa A; Zernecke, Alma 
Title:
Establishment of a new murine elastase-induced aneurysm model combined with transplantation. 
Abstract:
The aim of our study was to develop a reproducible murine model of elastase-induced aneurysm formation combined with aortic transplantation.Adult male mice (n = 6-9 per group) underwent infrarenal, orthotopic transplantation of the aorta treated with elastase or left untreated. Subsequently, both groups of mice were monitored by ultrasound until 7 weeks after grafting.Mice receiving an elastase-pretreated aorta developed aneurysms and exhibited a significantly increased diastolic vessel diameter compared to control grafted mice at 7 week after surgery (1.11 ± 0.10 mm vs. 0.75 ± 0.03 mm; p<= 0,001). Histopathological examination revealed disruption of medial elastin, an increase in collagen content and smooth muscle cells, and neointima formation in aneurysm grafts.We developed a reproducible murine model of elastase-induced aneurysm combined with aortic transplantation. This model may be suitable to investigate aneurysm-specific inflammatory processes and for use in gene-targeted animals. 
Journal title abbreviation:
PLoS ONE 
Year:
2014 
Journal volume:
Journal issue:
Pages contribution:
e102648 
Language:
eng 
TUM Institution:
Klinik für Gefäßchirurgie