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Dokumenttyp:
journal article 
Autor(en):
Adelsberger, Helmuth; Grienberger, Christine; Stroh, Albrecht; Konnerth, Arthur 
Titel:
In vivo calcium recordings and channelrhodopsin-2 activation through an optical fiber. 
Abstract:
We describe here an approach for the fluorometric monitoring of population activity in neurons in live mice combined with the activation of optogenetic actuators in vivo. In this protocol, a thin multimode fiber, which is used for both delivering excitation light and collecting emitted fluorescence signals, is inserted into the skull of a mouse. When combined with multicell bolus loading of Ca(2+) indicators, this optical fiber and its associated fluorescence detection system can be used for the...    »
 
Zeitschriftentitel:
Cold Spring Harb Protoc 
Jahr:
2014 
Band / Volume:
2014 
Heft / Issue:
10 
Seitenangaben Beitrag:
pdb.prot084145 
Sprache:
eng 
Print-ISSN:
1940-3402 
TUM Einrichtung:
Institut für Neurowissenschaften