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Dokumenttyp:
journal article 
Autor(en):
Chapman, Sean, S; Stevens, Laura J, LJ; Boevink, Petra C, PC; Engelhardt, Stefan, S; Alexander, Colin J, CJ; Harrower, Brian, B; Champouret, Nicolas, N; McGeachy, Kara, K; Van Weymers, Pauline S M, PS; Chen, Xinwei, X; Birch, Paul R J, PR; Hein, Ingo, I 
Titel:
Detection of the virulent form of AVR3a from Phytophthora infestans following artificial evolution of potato resistance gene R3a. 
Abstract:
Engineering resistance genes to gain effector recognition is emerging as an important step in attaining broad, durable resistance. We engineered potato resistance gene R3a to gain recognition of the virulent AVR3aEM effector form of Phytophthora infestans. Random mutagenesis, gene shuffling and site-directed mutagenesis of R3a were conducted to produce R3a* variants with gain of recognition towards AVR3aEM. Programmed cell death following gain of recognition was enhanced in iterative rounds of a...    »
 
Zeitschriftentitel:
PLoS ONE 
Jahr:
2014 
Band / Volume:
Heft / Issue:
10 
Seitenangaben Beitrag:
e110158 
Sprache:
eng 
TUM Einrichtung:
r Pharmakologie und Toxikologie