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Author(s):
Fend, Falko; Kremer, Marcus; Quintanilla-Martinez, Leticia 
Title:
Laser Capture Microdissection: Methodical Aspects and Applications with Emphasis on Immuno-Laser Capture Microdissection 
Abstract:
Laser capture microdissection (LCM) is an easy, extremely fast and versatile method for the isolation of morphologically defined cell populations from complex primary tissues for molecular analyses. However, the optical resolution is limited due to the use of dried sections without coverslip necessary for tissue capture, and routine stains such as hematoxylin and eosin are sometimes insufficient for precise microdissection, especially in tissues with diffuse intermingling of different cell types...    »
 
Keywords:
Laser capture microdissection; Immunohistochemistry; RT-PCR; Gene expression; Polymerase chain reaction 
Journal title:
Pathobiology 
Year:
2000 
Journal volume:
68 
Journal issue:
4-5 
Pages contribution:
209--214 
Publisher:
S. Karger AG 
Publisher address:
Basel, Switzerland 
Print-ISSN:
1423-0291 
E-ISSN:
1423-0291 
Notes:
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.