Schlitzer, Andreas; McGovern, Naomi; Teo, Pearline; Zelante, Teresa; Atarashi, Koji; Low, Donovan; Ho, Adrian W S; See, Peter; Shin, Amanda; Wasan, Pavandip Singh; Hoeffel, Guillaume; Malleret, Benoit; Heiseke, Alexander; Chew, Samantha; Jardine, Laura; Purvis, Harriet A; Hilkens, Catharien M U; Tam, John; Poidinger, Michael; Stanley, E Richard; Krug, Anne B; Renia, Laurent; Sivasankar, Baalasubramanian; Ng, Lai Guan; Collin, Matthew; Ricciardi-Castagnoli, Paola; Honda, Kenya; Haniffa, Muzlifah; Ginhoux, Florent     «

Schlitzer, Andreas; McGovern, Naomi; Teo, Pearline; Zelante, Teresa; Atarashi, Koji; Low, Donovan; Ho, Adrian W S; See, Peter; Shin, Amanda; Wasan, Pavandip Singh; Hoeffel, Guillaume; Malleret, Benoit; Heiseke, Alexander; Chew, Samantha; Jardine, Laura; Purvis, Harriet A; Hilkens, Catharien M U; Tam, John; Poidinger, Michael; Stanley, E Richard; Krug, Anne B; Renia, Laurent; Sivasankar, Baalasubramanian; Ng, Lai Guan; Collin, Matthew; Ricciardi-Castagnoli, Paola; Honda, Kenya; Haniffa, Muzlifah;...     »

Mouse and human dendritic cells (DCs) are composed of functionally specialized subsets, but precise interspecies correlation is currently incomplete. Here, we showed that murine lung and gut lamina propria CD11b+ DC populations were comprised of two subsets: FLT3- and IRF4-dependent CD24(+)CD64(-) DCs and contaminating CSF-1R-dependent CD24(-)CD64(+) macrophages. Functionally, loss of CD24(+)CD11b(+) DCs abrogated CD4+ T cell-mediated interleukin-17 (IL-17) production in steady state and after Aspergillus fumigatus challenge. Human CD1c+ DCs, the equivalent of murine CD24(+)CD11b(+) DCs, also expressed IRF4, secreted IL-23, and promoted T helper 17 cell responses. Our data revealed heterogeneity in the mouse CD11b+ DC compartment and identifed mucosal tissues IRF4-expressing DCs specialized in instructing IL-17 responses in both mouse and human. The demonstration of mouse and human DC subsets specialized in driving IL-17 responses highlights the conservation of key immune functions across species and will facilitate the translation of mouse in vivo findings to advance DC-based clinical therapies.     «

Mouse and human dendritic cells (DCs) are composed of functionally specialized subsets, but precise interspecies correlation is currently incomplete. Here, we showed that murine lung and gut lamina propria CD11b+ DC populations were comprised of two subsets: FLT3- and IRF4-dependent CD24(+)CD64(-) DCs and contaminating CSF-1R-dependent CD24(-)CD64(+) macrophages. Functionally, loss of CD24(+)CD11b(+) DCs abrogated CD4+ T cell-mediated interleukin-17 (IL-17) production in steady state and after A...     »