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Document type:
Journal Article; Research Support, Non-U.S. Gov't 
Author(s):
Bonnas, C; Specht, K; Spleiss, O; Froehner, S; Dietmann, G; Krüger, JM; Arbogast, S; Feuerhake, F 
Title:
Effects of cold ischemia and inflammatory tumor microenvironment on detection of PI3K/AKT and MAPK pathway activation patterns in clinical cancer samples. 
Abstract:
The accuracy of common markers for PI3K/AKT and MAPK pathway activation in preclinical and clinical cancer biomarker studies depends on phosphoepitope stability and changes of phosphorylation under ischemia. Herein, we define conditions under which phosphoepitope-specific duplex immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tumor tissues reflects pathway activation in situ as accurately as possible, and identify activation patterns linked to mutational status, pathway dependenc...    »
 
Journal title abbreviation:
Int J Cancer 
Year:
2012 
Journal volume:
131 
Journal issue:
Pages contribution:
1621-32 
Language:
eng 
Fulltext / DOI:
Print-ISSN:
0020-7136 
TUM Institution:
Institut für Allgemeine Pathologie und Pathologische Anatomie