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Document type:
journal article 
Author(s):
Peters, R; Wolf, MJ; van den Broek, M; Nuvolone, M; Dannenmann, S; Stieger, B; Rapold, R; Konrad, D; Rubin, A; Bertino, JR; Aguzzi, A; Heikenwalder, M; Knuth, AK 
Title:
Efficient generation of multipotent mesenchymal stem cells from umbilical cord blood in stroma-free liquid culture. 
Abstract:
Haematopoiesis is sustained by haematopoietic (HSC) and mesenchymal stem cells (MSC). HSC are the precursors for blood cells, whereas marrow, stroma, bone, cartilage, muscle and connective tissues derive from MSC. The generation of MSC from umbilical cord blood (UCB) is possible, but with low and unpredictable success. Here we describe a novel, robust stroma-free dual cell culture system for long-term expansion of primitive UCB-derived MSC.UCB-derived mononuclear cells (MNC) or selected CD34(+)...    »
 
Journal title abbreviation:
PLoS ONE 
Year:
2010 
Journal volume:
Journal issue:
12 
Pages contribution:
e15689 
Language:
eng 
TUM Institution:
Institut für Virologie